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. 2021 Mar 19;22(6):3158. doi: 10.3390/ijms22063158

Figure 2.

Figure 2

(A) PDK−4, (B) AngPtl4 and (C) iNOS mRNA expression in pulmonary arteries following incubation with LPS and PPARβ/δ ligands. The expression of different mRNA was measured following 24 h incubation with treatments: vehicle (0.01% DMSO); 1 µg/mL LPS; 1 µg/mL LPS + 100 nM GW0742; 1 µg/mL LPS + 1 µM GSK3787; and 1 µg/mL LPS + 100 nM GW0742 + 1 µM GSK3787 (n = 4–5). Relative quantitation was calculated with the comparative CtΔΔ method and normalized against β-actin as an endogenous control. The data are presented as mean ± standard deviation; the data was not normally distributed (D’Agostino–Pearson normality test). Significant difference by the Krustal–Wallis test with Dunns post hoc test is indicated by * = p < 0.05, ** = p < 0.01 and *** = p < 0.001 compared with Vehicle; f = p < 0.05, ff = p < 0.01 compared to LPS; ## = p < 0.01 and ### = p < 0.001 compared to LPS + GW0742.