Figure 4.

Whole-cell patch-clamp recordings. (a) Voltage-current plots before (control—green dots) and after application of 16 µM capsaicin (CAP—red dots) and 20 µM of capsazepine (CPZ—blue dots). Presence of CAP increased the ion currents. Subsequent perfusion with CPZ lower CAP-induced ion currents to a level similar to the control. (b) Current density of control, CAP-, and CPZ-perfused cells at −80mV. A significant increase in current density was observed in the CAP-treated cells in comparison to control and CPZ. Significance was denoted with an asterisk (*). Values expressed as mean ± s.e.m., n = 6, one-way ANOVA, with Tukey’s post hoc test (p < 0.05).