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. 2021 Mar 20;22(6):3188. doi: 10.3390/ijms22063188

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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M.C increased the ratio of M2a and M2c macrophages. Spleen-derived cells from the sarcopenia mouse model were assessed by flow cytometry. (A,B) Representative flow cytometry (FACS) dot plots of CD206, CD163 in CD11b⁺F4/80⁺, and CD45⁺cells. CD206⁻CD163⁻ M1 macrophages; CD206⁺CD163⁻ M2a macrophages; CD206⁺CD163⁺M2c macrophages; (C–E) graphs of M1 macrophage, M2a macrophage, and M2c macrophage, presented as % of CD11b⁺F4/80⁺ cells; (F) the CD4⁺/CD8 ratio of CD45⁺ cells in splenocytes, presented as the mean ± SEM (n = 4/group). * p < 0.05, ** p < 0.01, *** p < 0.001 vs. CIS+PBS, using a Bonferroni post hoc test after one-way ANOVA.