Figure 5.

ALLO modifies the motility of BV-2 cells grown in a low concentration of serum. (A) The path length represents the total path traveled by the cells during the experiment (black line). D2S is the distance between the start and the end point (dotted blue line). (B) The path length and (C) the distance traveled between the first and the last position of the BV-2 cells (D2S length) were measured from time-lapse imaging. (D) The directionality (i.e., the ratio D2S length/path length), (E) the mean velocity and (F) the maximal velocity of BV-2 cells were subsequently determined. BV-2 cells were grown in 1% FCS. Mean values ± SEM are shown, with n = 146 control cells and n = 129 ALLO-treated cells, from four independent experiments, ** p < 0.01, *** p < 0.001.