Figure 4.

Genetic disruption of the ASCT2 cysteine transporter affects cooperation between xCT-expressing and xCT^KO cells. (A). A variety of xCT wt cells (LS174T, A549, MiaPaCa-2, Capan-2 and HDF) were grown in DMEM media +/− amino acids during 24 h and protein content of different cysteine transporters (ASCT1 (SLC1A4), ASCT2 (SLC1A5), EAAT3 (SLC1A1), EAAT4 (SLC1A6) were analyzed by Western blotting. (B,C). Lipid hydroperoxide accumulation and cell viability of MiaPaCa-2 xCT^KO and xCT-ASCT2^DKO cells (guest cells—CySH import) in control conditions or co-cultured with 6% A549 wt, ASCT1^KO, ASCT2^KO, ASCT1-ASCT2^DKO, or LAT1^KO (host cells—CySH export). All experiments have been performed in triplicate and the representative blots and histograms are shown. Bar graph shows mean ± SEM; n = 3; *, p < 0.05, comparison with WT control group.