Figure 8.

Heterotypic henipavirus F and G complementation assay confirms MojV F functionality in a quantitative Nano luciferase reporter cell–cell fusion assay. BSR-T7/5 effector cells were co-transfected with empty vector or henipavirus F and either (A) HeV G, (B) NiV G, (C) CedV G, (D) GhV G or (E) MojV G. Effector cells were co-incubated with C6 rat glial cells transfected with the pTM1-Nluc reporter plasmid. Maximum RLUs were recorded 48 h post-application of target cells to all effector monolayers, except for co-cultures with effector cells expressing MojV G, which reached maximum RLUs on day 4. Maximum RLUs were normalized against background defined as RLUs from mixtures of the target C6 cells applied to effector cells co-transfected with henipavirus G and empty vectors. The experiments were performed at least 3 times in technical triplicates. A representative experiment is shown here. Data were analyzed by Welch’s t test. The error bars represent the standard deviation. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.