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. 2021 Mar 22;19(3):171. doi: 10.3390/md19030171

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Purification and composition identification of EPS364. (A) The chromatograph profile of the crude polysaccharide EPS364 on a DEAE Sepharose Fast Flow column eluted with a linear gradient of NaCl aqueous solutions (0–1.0 M) at a flow rate of 2.0 mL/min. (B) The elution profile of EPS364 on a Sephadex G-100 column eluted with ultrapure water at a flow rate of 1.0 mL/min. (C) UV spectrum of EPS364 detected by a NanoPhotometer^® spectrophotometer. (D) HPGPC (high-performance gel permeation chromatography) profile of EPS364. (E) The monosaccharide composition of EPS364. Abbreviations: Man, mannose; GlcN, glucosamine; Rha, rhamnose; GlcA, gluconic acid; GalA, galacturonic acid; GalN, galactosamine; Glc, glucose; Gal, galactose; Xyl, xylose and Ara, arabinose.