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. 2021 Mar 22;19(3):171. doi: 10.3390/md19030171

Figure 5.

Figure 5

EPS364 induced apoptosis, the loss of the mitochondrial membrane potential (MMP) and generation of reactive oxygen species (ROS) in Huh7.5 cells. After incubation with or without the EPS364 treatment (2 mg/mL) for 12 h, Huh7.5 cells were stained by Annexin V-FITC (fluorescein isothiocyanate) and PI (propidium iodide) and detected by a flow cytometry. (A) Representative dot plots of Annexin V/PI staining. (B) Column bar graph of apoptotic cells. (C) EPS364 induced the loss of the MMP in Huh7.5 cells with 12 h of treatment. (D) EPS364 induced the generation of ROS in Huh7.5 cells with 12 h of treatment. * p < 0.05 and ** p < 0.01 versus the control.