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HiBiT-RBD is expressed in both cell lysates and supernatants. (A) HEK293T cells were transiently transfected with HiBiT-RBD or pcDNA3.1 empty vector control (1 μg total). Harvested whole cell lysates and supernatants were immunoblotted and probed for RBD. Beta-actin was used as a loading control, and Ponceau staining shows total protein levels. (B) Relative luminescence signal with and without LgBiT-substrate treatment of both cell lysates and supernatants.
