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. 2021 Mar 22;7(3):236. doi: 10.3390/jof7030236

Table 1.

Activity of antioxidative enzymes and TBARS content under drought (PEG), fullerol C60(OH)24 nanoparticles (FNP; 0, 10, 100, and 1000 ng mL−1) and combined FNP–PEG treatments (10 ng mL−1 FNP + PEG, 100 ng mL−1 FNP + PEG, and 1000 ng mL−1 FNP + PEG).

Treatment APX CAT POD PPO TBARS [pmol/g]
(nkat/g DW)
Control; 0 ng mL−1 FNP 0.1 ± 0.1 bc 152.9 ± 12.2 ab 3.3 ± 0.4 d 1.0 ± 0.0 a 6.2 ± 2.1 c
PEG 0.8 ± 0.1 bcd 168.3 ± 27.5 a 4.5 ± 0.9 c 1.1 ± 0.0 a 57.7 ± 6.6 a
10 ng mL−1 FNP 0.6 ± 0.08 de 165.1 ± 24.4 a 2.9 ± 0.0 d 1.1 ± 0.0 a 18.6 ± 3.5 c
100 ng mL−1 FNP 0.6 ± 0.1 cde 110.1 ± 0.0 bc 4.4 ± 0.7 c 0.9 ± 0.1 a 51.3 ± 1.0 ab
1000 ng mL−1 FNP 1.1 ± 0.0 b 99.7 ± 13.7 cd 6.1 ± 0.1 b 1.2 ± 0.3 a 44.6 ± 6.2 b
10 ng mL−1 FNP+ PEG 0.3 ± 0.0 e 89.7 ± 9.3 cd 6.2 ± 0.6 b 1.1 ± 0.0 a 56.8 ± 0.2 a
100 ng mL−1 FNP+ PEG 0.7 ± 0.0 bcd 63.0 ± 9.4 de 3.1 ± 0.3 d 1.0 ± 0.1 a 67.1 ± 1.0 a
1000 ng mL−1 FNP+ PEG 1.9 ± 0.3 a 30.6 ± 6.1 e 7.4 ± 0.2 a 1.1 ± 0.0 a 74.9 ± 12.2 a

Values are means ± S.D. (n = 3). The different letters indicate statistically significant differences between treatments at p < 0.05, using Tukey’s post-hoc test. APX—ascorbate peroxidase (EC 1.11.1.11); CAT—catalase (EC 1.11.1.6); POD—nonspecific peroxidase (EC 1.11.1.7); PPO—polyphenol oxidase (EC 1.14.18.1); TBARS—thiobarbituric acid reactive substances.