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. 2021 Mar 22;13(3):1022. doi: 10.3390/nu13031022

Figure 3.

Figure 3

Pretreatment with DPPS attenuates markers of liver damage in ConA-induced acute hepatitis. DPPS pretreatment shows hepatoprotective effects in ConA-induced hepatitis. C57BL/6 mice were intravenously injected with 12.5 mg/kg of Concanavalin A. DPPS was administered orally (20 µL of 50% mixture solution), six hours before ConA injection. Livers were analyzed 12 and 24 h after ConA injection. (a) Representative H&E staining of paraffin-embedded liver sections, magnification 100×. (b) ALT levels determined in the serum 0, 8, 10, 18, and 30 h after oral administration of DPPS. (c) Serum levels of ALT determined 2, 4, 12, and 24 h after ConA injection (n = 7). (d) TUNEL staining of liver sections 6 h after ConA injection, magnification 400×. (e) Quantitative analysis of cell death rate: TUNEL-positive nuclei (brown) were counted in five random fields, and the data were summarized as the mean number of positive cells+SD (*** p < 0.001; ** p < 0.005; * p < 0.05; two-tailed, unpaired Student’s t-test).