Figure 5.

Second-generation deacetylase plus BET inhibition. (A) Chemical structures and (B) 50% growth inhibition (IC₅₀) values in colon cancer cells treated with bromodomain and extraterminal domain (BET) inhibitors for 48 h; mean ± SE * p < 0.05, ** p < 0.01 (metastatic vs. primary). (C) Combination index (CI) data for SW620 cells in 2D monolayers treated with BG45 + dBET6, as in panel B. (D) Representative images of SW620 3D spheroids on day 3 after seeding 5000 cells/well. (E) CI data plotted for SW620 3D cells treated as in C. (F) Immunoblot analysis after SW620 3D spheroids were treated with Vehicle, BG45 (50 µM), dBET6 (1.56 µM), or BG45 + dBET6 for 6 and 48 h. β-Actin served as a loading control.