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. 2021 Mar 22;13(3):424. doi: 10.3390/pharmaceutics13030424

Figure 4.

Figure 4

Microbial and fungal sterility of nGO and nGO-PEG nanoparticles suspensions. Pristine GO and nGO-PEG nanosheets were streaked on three different types of agar plates: Columbia agar with 5% Sheep Blood (Becton Dickinson) for Gram (+) and Gram (–) microorganisms; MacConkey agar (Becton Dickinson)—a selective and differentiating agar for Gram (–) bacterial species, and Sabouraud Dextrose Agar (Becton Dickinson)—a nonselective medium for fungi, particularly Staphylococcus aureus, Escherichia coli and Candida albicans were used as positive controls of bacterial and fungal growth. Agar plates were incubated for 72 h at 37°C. Microphotographs were taken with a Nikon D700 reflex camera (Nikon, Tokyo, Japan).