Table 1.
Experimental setting
| Sample | Sample group | Sample collection | Sample handling | Sample input | DNA extraction |
|---|---|---|---|---|---|
| Human |
OB, n= 19 (n= 9 PD, n= 10 HC) |
Non- sterile | Sterile | Brain tissue | Proteinase K |
|
GFI, n= 20 (n= 10 PD, n= 10 HC) |
Sterile | ||||
|
PN, n= 8 (n= 6 PD, n= 2 HC) |
Non-sterile | ||||
|
Cortex, n= 3 (SC) |
Sterile | Sterile | |||
| Murine |
SPF, n=5 GRF, n=3 |
Non- sterile | Sterile | Brain tissue | Proteinase K |
| Sterile | |||||
| Controls | DNA extraction blank control (KitUKB), n= 3 | NA | Sterile | Empty | Proteinase K |
| Negative control (BF), n= 4 | NA | Sterile | Tris-HCl buffer | Proteinase K | |
| Negative control (SW), n= 3 | NA | Sterile | Sterile water | Proteinase K | |
| Positive DNA extraction control (mock community), n= 6 | NA | Sterile | Serially diluted mock community | Proteinase K | |
| No-template control (KitQIB), n= 8 | NA | Sterile | PCR reagents | No |
OB olfactory bulb, GFI frontal inferior cortex, PN putamen, SPF specific pathogen free, GRF germ free, BF Tris-HCl buffer, SW sterile/autoclaved water, KitUKB kitome UKB, empty tube, KitQIB kitome QIB, PCR reagents, NA not applicable