Fig. 4.
Expression of PDL-1 and SIRP-1α are increased in DC treated with 2-DG (2-DGtolDC). a Percentage of PDL-1+ DC, with or without 2-DG (2.5 mM over 24 h), and/or, stimulated with heat-inactivated mycobacterial extract (Mtb, 15 μg/ml over 24 h) (gated on live, singlet, CD11bhiCD11chi cells). Treatments are indicated by the ± matrix below graph. b Percentage of PDL-1+ DC treated with 2-DG for 1/6/24 h. Below, histograms of PDL-1 expression intensity (MFI) for each time point are shown, with untreated DC in blue, and 2-DGtolDC in red. Note increase in the intensity of PDL-1 expression in 2-DGtolDC. c Percentage of DC in live, singlet CD11bhiCD11chi gate, that are positive for SIRP-1α, DC treatments/stimulations are indicated in the ± matrix. d Percentage of DC, treated with 2-DG for 1/6/24 h, positive for SIRP-1α. Below, histograms of SIRP-1α MFI for each time point are shown, with untreated DC in blue, and 2-DGtolDC in red. Error bars denote Standard Error of the Mean (SEM), p values: * < 0.05, ** < 0.01, *** < 0.001, n = 3