Fig. 3.

Myeloproliferation in miR-146a^−/− mice is not affected by high-fat diet. After 10 weeks of high-fat diet (HFD) or respective normal diet (ND), miR-146a^−/− (KO) or respective control mice (WT) were sacrificed and splenocytes were isolated and analysed by flow cytometry. a Spleen weight relative to body weight. b T cells (CD3⁺) and c B cells (CD19⁺). d Gating strategy for myeloid-derived subpopulations. e Myeloid cells (Mye), f CD11b⁺/c⁺ I-Ab^low cells, g CD11b⁺/c⁺ dendritic cells (DCs), h neutrophils (Neu), i macrophages (Mac), and j eosinophils (Eos). Data are displayed as mean and SEM of 5 animals per group. Statistics: (a–c, e–j) one-way ANOVA with Tukey correction. *p < 0.05, **p < 0.01, ***p < 0.001