Fig. 5.

miR-146a regulates insulin-stimulated glucose uptake in adipocytes. To assess the influence of miR-146a on insulin sensitivity in vitro, glucose uptake experiments were performed with SGBS adipocytes. The amount of glucose taken up by the cells was measured by scintillation counting and normalized to the basal glucose uptake (0 nM insulin) and phosphorylation of AKT was assessed. a miR-146a inhibitor (50 nm) or inhibitor control (NTi, 50 nM) transfected adipocytes. b miR-146a mimic (20 nM) or control (NT, 20 nM) transfected adipocytes. c Adipocytes stably overexpressing miR-146a or non-target control (Ctrl). d Representative Western blot for pAKT (Ser473) and AKT after 15 min stimulation with 0 nM and 10 nM insulin with α-tubulin as loading control. e Densitometric analysis of 4 independent experiments displayed as mean and SEM with phosphorylated AKT (Ser473) normalized to total AKT. Data are displayed as mean and SEM of 3 (a and c) or 4 (b) independent experiments. Statistics: (a, b, c) two-way ANOVA with Bonferroni correction and non-linear fit with four parameters, (e) paired t test, *p < 0.05, **p < 0.01