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. 2021 Mar 22;10(6):1308. doi: 10.3390/jcm10061308

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Sensitivity of the genus-specific LAMP assay in simulated human urine samples artificially spiked with gDNA from different hybrid schistosomes. (A) Sensitivity assessment of LAMP when performing a simple heating method from serial dilutions of hybrid schistosomes gDNA. (B) Sensitivity assessment of LAMP when performing the DNA extraction with the i-genomic Urine DNA Extraction Mini Kit (Intron Biotechnology, UK) from serial dilutions of hybrid schistosomes gDNA. Lanes 20, 10 and 10⁻¹–10⁻⁵: 20 ng, 10 ng and 10-fold serial dilutions, respectively; Sh-Sb/Sb, Sb-Sh/Sb, Sb-Sh/Sh, Sh-Sh/Sb, and Sb-Sh/Sh^c: gDNA from hybrid schistosomes; N: negative controls (no DNA template).