Table 2.
Primer sets used in this work for Schisto-LAMP assays. For S. mansoni, S. haematobium, S. bovis and Schistosoma spp.: F3, forward outer primer; B3, backward outer primer; FIP, forward inner primer (comprising F1c and F2 sequences); BIP, backward inner primer (comprising B1c and B2 sequences); LF, loop forward primer; LB = loop backward primer. bp, base pairs.
| Schisto-LAMP | Primer Sets | Sequence 5′→3′ | Length (bp) | Ref. |
|---|---|---|---|---|
| S. mansoni | F3 | TTATCGTCTATAGTACGGTAGG | 22 | [45] |
| B3 | ATACTTTAACCCCCACCAA | 19 | ||
| FIP | GCCAAGTAGAGACACAAACATCTT-TGGGTAAGGTAGAAAATGTTGT | 47 | ||
| BIP | AGAAGTGTTTAACTTGATGAAGGGG-AAACAAAACCGAAACCACTA | 45 | ||
| S. haematobium | F3 | CTTTCTAAGCCCGCGATA | 18 | [44] |
| B3 | GCGCATTACACTTGGTCT | 18 | ||
| FIP | TACCCCTAACTTCGTGGTCTCC-CCCCCTTATTTTAGGGTGC | 41 | ||
| BIP | CTCCCTATATAACATGGCGAGTAAG-ACTATGAAATCAGTGTTTTTCGG | 48 | ||
| S. bovis | F3 | TTCATTGTTAGGTTGCGT | 18 | [42] |
| B3 | TCTATATTCTACTCTAATCCCTCT | 24 | ||
| FIP | TCAGTATCATCTCAAACATCACACT-AGTAGTATGTTCTGTCTTAAGTT | 48 | ||
| BIP | TTTGTAGTACCTCTGGTTTACATCA-TTCACTCTCAGACTCTACAT | 45 | ||
| LF | ACTTAGACCATGAACATCAACCTAT | 25 | ||
| LB | TACTAAGTGAGAGTAATCGAACACC | 25 | ||
| Schistosoma spp. | F3 | TTGACCGGGGTACCTAGC | 18 | [42] |
| B3 | CGTGAATGGCAAGCCAAAC | 19 | ||
| FIP | ATCGCCCTTGGCAGATCAGG-CTGTCGTATGCCCTGATGG | 39 | ||
| BIP | ATATGCATGCAAATCCGCCCCG-CGGATCGCTTCAACAGTGTA | 43 | ||
| LF | CAGATCAGGCAACCCGAAAG | 22 |