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. 2021 Mar 22;13(6):1443. doi: 10.3390/cancers13061443

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Time-lapse microscopy analysis of NK-92/5.28.z cell activity over 18 h. Images of established RH30 cell monolayers were recorded during coincubation with NK-92/5.28.z (A) or parental NK-92 (B) cells (n = 1). NK-92/5.28.z cells proliferated and almost completely lysed RH30 cell monolayers, while NK-92 cells decreased in number and were not able to lyse RH30 cell monolayers or inhibit tumor growth. The observed effects are shown as exact cell counts (C). Here target and effector cells were discriminated by their different microscopic morphology as well as by their different culture behaviors (see Section 2.5).