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. 2021 Mar 22;22(6):3225. doi: 10.3390/ijms22063225

Table 1.

Increased yield of mutant lines in T0, T1, and T2 generations.

Generation Genotypes PH PN FLL FLW GNPP GL GWD GWT
T0 WT 145.3 ± 3.6 9.5 ± 1.6 60.2 ± 2.3 2.6 ± 0.3 148.6 ± 0.9 8.7 ± 0.2 2.9 ± 0.1 31.3 ± 1.3
GXU27-3 144.6 ± 4.2 ns 9.7 ± 2.2 ns 59.9 ± 3.1 ns 2.5 ± 0.5 ns 147.4 ± 0.8 ns 11.1 ± 0.3 * 3.0 ± 0.3 ns 39.8 ± 1.2 *
GXU27-4 146.4 ± 5.6 ns 9.6 ± 1.4 ns 60.3 ± 2.2 ns 2.6 ± 0.4 ns 146.5 ± 0.7 ns 10.8 ± 0.5 * 2.9 ± 0.2 ns 39.1 ± 1.6 *
GXU27-9 145.9 ± 4.8 ns 9.7 ± 1.8 ns 59.8 ± 2.5 ns 2.7 ± 0.3 ns 149.2 ± 0.8 ns 10.9 ± 0.2 * 2.9 ± 0.1 ns 39.0 ± 1.4 *
T1 WT 146.5 ± 2.8 9.6 ± 1.5 61.3 ± 2.6 2.5 ± 0.2 149.4 ± 0.6 8.8 ± 0.3 3.0 ± 0.1 32.1 ± 1.1
GXU27-3 145.4 ± 3.4 ns 9.8 ± 1.9 ns 60.2 ± 2.4 ns 2.7 ± 0.2 ns 148.5 ± 0.9 ns 11.3 ± 0.2 * 3.1 ± 0.1 ns 39.7 ± 1.4 *
GXU27-4 147.3 ± 4.2 ns 9.8 ± 1.2 ns 59.9 ± 2.5 ns 2.4 ± 0.5 ns 148.2 ± 0.5 ns 10.9 ± 0.3 * 3.0 ± 0.2 ns 39.3 ± 1.0 *
GXU27-9 145.8 ± 3.5 ns 9.6 ± 1.4 ns 60.1 ± 2.2 ns 2.6 ± 0.4 ns 149.6 ± 0.4 ns 10.6 ± 0.4 * 2.9 ± 0.2 ns 39.2 ± 1.2 *
T2 WT 144.6 ± 2.8 9.8 ± 1.3 59.9 ± 2.4 2.7 ± 0.2 148.2 ± 0.6 8.6 ± 0.3 2.9 ± 0.2 31.6 ± 1.2
GXU27-3 144.8 ± 3.7 ns 9.6 ± 2.0 ns 60.1 ± 3.2 ns 2.6 ± 0.3 ns 145.4 ± 0.6 ns 11.2 ± 0.4 * 3.0 ± 0.1 ns 39.6 ± 1.5 *
GXU27-4 145.9 ± 3.5 ns 9.8 ± 1.6 ns 60.0 ± 2.3 ns 2.6 ± 0.4 ns 147.5 ± 0.5 ns 10.9 ± 0.3 * 3.0 ± 0.1 ns 39.3 ± 1.2 *
GXU27-9 145.3 ± 4.2 ns 9.9 ± 1.7 ns 59.9 ± 2.4 ns 2.5 ± 0.2 ns 149.3 ± 0.7 ns 10.7 ± 0.2 * 2.9 ± 0.3 ns 39.1 ± 1.3 *

WT (wild type); PH (plant height) cm; PN (panicle numbers); FLL (flag leaf length) cm; FLW (flag leaf width) cm; GNPP (grain number per panicle); GL (grain length) mm; GWD (grain width) mm; GWT (1000-grain weight) g. Five independent plants were used to collect data from three replicates (n = 5). For grain phenotyping, five grains from each plant were selected randomly. * and ns denote the significant and non-significant differences (Student’s t-test, p < 0.01), respectively.