Figure 7.

Ligand binding properties of caMC4R. HEK293T cells were transiently transfected with MC4R plasmids, and the binding properties were measured 48 h later by displacing the binding of ¹²⁵I-NDP-MSH using different concentrations of unlabeled NDP-MSH (A), ACTH (1–24) (B), α-MSH (C), or β-MSH (D) as described in Materials and Methods. Data were expressed as % of hMC4R binding ± range in the absence of competitor from duplicate measurements within one experiment. All experiments were performed at least three times independently.