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. 2021 Mar 23;22(6):3269. doi: 10.3390/ijms22063269

Figure 1.

Figure 1

Kirenol (KRL)-attenuated DOX-induced cytotoxicity in H9c2 cardiac cells. (A) Chemical structure of kirenol. (B,C) Effect of KRL on the viability of H9c2 cardiomyocytes examined by an MTT assay. H9c2 cells were treated with increasing concentrations of KRL (2.5–20 μmol) for 24 and 48 h, respectively. Cell viability (%) was measured as follows: (A570 of treated cells/A570 of untreated cells) × 100. (D,E) The cell viability with increasing concentrations of DOX (0.1–1 μmol) for 24 and 48 h, respectively. (F,G) The effects of KRL along with DOX on the cell viability of H9c2 cells were determined by MTT assay. H9c2 cells were cultured in serum free media for 3 h followed by the treatment with KRL for 2 h before or after DOX treatment, respectively. Data are represented as the mean ± SD of triplicate values (n = 3) and * p < 0.05 represents significant variations compared with the control. # p < 0.05 represents significant variations as compared to DOX alone and KRL with DOX treatment groups.