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. 2021 Mar 23;22(6):3269. doi: 10.3390/ijms22063269

Figure 2.

Figure 2

Effects of KRL on DOX-induced hypertrophy. H9c2 cells were treated for 24 h with 15 µM of KRL, 2 h before DOX (0.25 µmol) treatment. (A) Representative Western blots showing changes in the protein levels of ANP and BNP. (B) KRL is illustrated to downregulate MMP9 and MMP2. Western blot analysis was performed to determine the total protein MMP9 and MMp2 levels in the total extract by including β-actin as an internal loading control. (C) H9c2 cells were treated for 24 h with 10 and 15 µM of KRL, 2 h before the DOX (0.25 µmol) treatment. The cells then underwent actin filament staining to observe the changes in the surface area of the cardiomyocytes. Scale bar indicated 100µ m at 20× magnification. Data are represented as the mean ± SD of triplicate values (n = 3) and * p < 0.05 represents significant variations compared with the control. # p < 0.05 represents significant variations as compared to DOX alone and KRL with DOX treatment groups.