Figure 3.

Sequence alignments of the N-terminal regions of plant APYs. The multisequence alignment was generated using Clustal Omega [55]. TOPCONS webserver [54] predictions are shown in bold. The predicted single-pass outward-facing transmembrane helix in the AtAPY1 sequence is highlighted in green. Bold red indicates predicted secretion peptide sequences. Relative positions of these N-terminal sequences to the ACR1 sequence motif (green box) are shown, the latter of which is present in the globular portion of all homology models and crystal structures.