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(A) Assay development indicated treatment with 100 ug/mL heparin was the minimum concentration required for effective removal of all outer-associated siRNA. (B) Heparin displacement assays indicated siRNA complexation to R8-PLP surface (panel 1), total EE% (panel 2), and siRNA entrapment within the R8-PLP core (panels 3 and 4), in a lipid:siRNA dependent manner.
