Table 3.
Liposome characterization related to R8 modification and assembly conditions.
| Modification Conditions | Pre-Modification | Post-Modification | ||||||
|---|---|---|---|---|---|---|---|---|
| R8-Lipid Conjugate |
Modification Type | Size (d.nm) | PDI | ζPotential (mV) | Size (d.nm) | PDI | ζPotential (mV) | |
| PLP | N/A | N/A | 55.84 ± 5.00 | 0.25 ± 0.04 | 9.74 ± 1.02 | 55.84 ± 5.00 | 0.25±0.04 | 9.74 ± 1.02 |
| R8-PLP | R8-PEG | Pre-insertion | N/A | N/A | N/A | 820.8 ± 375.7 | 0.52 ± 0.13 | 9.93 ± 1.44 |
| R8-PEG | Post-insertion (4 °C) | 58.32 ± 1.75 | 0.18 ± 0.012 | 10.21 ± 1.16 | 68.01 ± 5.89 | 0.217 | 10.72 ± 2.15 | |
| R8-PEG | Post-insertion (37 °C) | 61.75 ± 3.17 | 0.22 ± 0.02 | 10.70 ± 1.48 | 72.55 ± 5.45 | 0.180 | 11.92 ± 1.45 | |
| R8-PEG | Post-conjugation (4 °C) | 59.70 ± 6.14 | 0.2 1 ± 0.05 | 10.44 ± 1.44 | 176.78 ± 47.89 | 0.313 | 10.64 ± 1.27 | |
| R8-PEG | Post-conjugation(37 °C) | 55.98 ± 5.47 | 0.21 ± 0.04 | 10.58 ± 1.49 | 172.4 ± 52.1 | 0.354 | 9.11 ± 0.94 | |
| STR-R8 | Pre-insertion | N/A | N/A | N/A | 49.65 ± 1.77 | 0.22 ± 0.01 | 7.40 ± 1.90 | |
| STR-R8 | Post-insertion (4 °C) | 51.14 ± 0.97 | 0.16 ± 0.01 | 1.54 ± 6.87 | 53.74 ± 2.37 | 0.21 ± 0.02 | 13.70 ± 2.63 | |
| STR-R8 | Post-insertion (37 °C) | 49.00 ± 1.75 | 0.21 ± 0.01 | 8.54 ± 2.35 | 48.83 ± 2.00 | 0.21 ± 0.01 | 12.10 ± 0.55 | |