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. 2021 Mar 23;22(6):3249. doi: 10.3390/ijms22063249

Figure 5.

Figure 5

Analysis of nuclear fraction from hypoxia-treated HL-1 cardiomyocytes that modulated by JNK transduction pathway. Panel A: Nuclear lysates were analyzed by Western blots to determine levels of HIF-1α, p-c-Jun, p-ATF2 in HL-1 cells under the control condition (no treatment), treatment with CoCl2 (10−4 M) for 6 h, or hypoxia (1% O2) for 6 h. The data are mean ± SEM (n = 4 per group, * p < 0.05 vs. control) from four independent experiments. Panel B: Representative Western blots and levels of HIF-1α, p-c-Jun, p-ATF2 in the nuclear lysate of HL-1 cells pre-treated with SP600125 (10−5 M) or DMSO as a vehicle for 30 min, then treated with hypoxia stress (1% O2) for 6 h. The bar graph shows the value of each sample relative to that of control cells under normoxia (vehicle only) or hypoxic condition. The data are mean ± SEM (n = 3 per group, * p < 0.05 vs. control; # p < 0.05 vs. hypoxia) from three independent experiments.