Figure 6.
Following persistent stimulation of MSCs with TNFα + IL-1β, cancer-relevant programs are promoted in the resulting inflammatory CAFs. Human MSCs were exposed to persistent TNFα + IL-1β stimulation (concentrations as in Figure 1) or to vehicles for 14 days in transcriptome analyses and for 18–19 days in secretome analyses. (A) Heat maps of protein expression determined by secretome analysis of three independent biological repeats, comparing cytokine-stimulated cells and control cells. (B) Disorder analysis of cancer-related genes and secreted proteins for which expression was modified in cytokine-stimulated cells compared to vehicle-treated cells. (B1) Gene expression data were obtained by RNAseq analysis, in which genes were filtered using a cutoff of FC > 2 or FC < 0.5, padj < 5 × 10−10. (B2) Data of secreted proteins were obtained by secretome analysis, in which proteins were filtered using a cutoff of FC ≥ 2 or FC ≤ 0.5, p < 0.05. The INGENUITY program was used to identify affected disorders. Indicated disorders obeyed the cutoff of p < 10−17 in transcriptome analyses and p < 5 × 10−8 in secretome analyses.