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Four-parameter logistic (4PL) curve-fit (calculated concentrations vs. raw optical density (OD) measurements) including 0.1, 0.4, 1.7, 7, 30 ng/mL cortisol standard concentrations (red diamonds), low and high controls (blue triangles) and blow sample data points (turquoise dots) for all ELISA results (n = 120). The three different curves correspond to the three assays that were run. As the actual sample concentrations were unknown, 1 was applied as dilution factor for modelling.
