Table 1.
Synopsis of applied staining protocols for frozen sections including each antibody setup.
| N° | Staining Protocol | Antibody Setup |
|---|---|---|
| ① | Quadruple staining of PLP, Calbindin D28k, MBP and Hoechst for Cerebellar Tissue | Primary Antibodies |
| Rat anti-PLP Guinea pig anti-Calbindin D28k Rabbit anti-MBP | ||
| Secondary Antibodies | ||
| Donkey anti-rat Alexa 488 Goat anti-guinea pig Alexa 555 Donkey anti-rabbit Alexa 647 Hoechst | ||
| ② | Quadruple staining of PLP, Calbindin D28k, GFAP and Hoechst for Cerebellar Tissue | Primary Antibodies |
| Rat anti-PLP Guinea pig anti-Calbindin D28k Rabbit anti-GFAP | ||
| Secondary Antibodies | ||
| Donkey anti-rat Alexa 488 Goat anti-guinea pig Alexa 555 Donkey anti-rabbit Alexa 647 Hoechst | ||
| ③ | Triple Staining of GFAP, Synaptophysin and α-BT for Skeletal Muscle and Esophagus | Primary Antibodies |
| Rabbit anti-GFAP Guinea pig anti-Synaptophysin | ||
| Secondary Antibodies and Toxins | ||
| Donkey anti-rabbit Alexa 488 Donkey anti-guinea pig Alexa 647 α-Bungarotoxin Alexa 555 | ||
| ④ | Triple staining of MBP, Synaptophysin and α-BT for Skeletal Muscle | Primary Antibodies |
| Rabbit anti-MBP Guinea pig anti-Synaptophysin | ||
| Secondary Antibodies and Toxins | ||
| Donkey anti-rabbit Alexa 488 Donkey anti-guinea pig Alexa 647 α-Bungarotoxin Alexa 555 | ||
| ⑤ | Quadruple staining of PLP, Synaptophysin, α-BT and Hoechst for Skeletal Muscle and Esophagus | Primary Antibodies |
| Rat anti-PLP Guinea pig anti-Synaptophysin | ||
| Secondary Antibodies and Toxins | ||
| Donkey anti-rat Alexa 488 Donkey anti-guinea pig Alexa 647 α-Bungarotoxin Alexa 555 Hoechst | ||
| ⑥ | Triple Staining of ChAT, MBP and α-BT for Skeletal Muscle | Primary Antibodies |
| Goat anti-ChAT Rabbit anti-MBP | ||
| Secondary Antibodies and Toxins | ||
| Donkey anti-goat Alexa 647 Donkey anti-rabbit Alexa 488 α-Bungarotoxin Alexa 555 | ||
| ⑦ | Triple Staining of ChAT, Synaptophysin and α-BT for Skeletal Muscle | Primary Antibodies |
| Goat anti-ChAT Guinea pig anti-Synaptophysin | ||
| Secondary Antibodies and Toxins | ||
| Donkey anti-goat Alexa 488 Donkey anti-guinea pig Alexa 647 α-Bungarotoxin Alexa 555 | ||
| ⑧ | Triple Staining of βIII-tubulin, MBP and α-BT for Skeletal Muscle | Primary Antibodies |
| Rabbit anti-βIII-tubulin Chicken anti-MBP | ||
| Secondary Antibodies and Toxins | ||
| Donkey anti-rabbit Alexa 488 Goat anti-chicken Alexa 647 α-Bungarotoxin Alexa 555 |