Skip to main content
. 2021 Mar 23;22(6):3285. doi: 10.3390/ijms22063285

Figure 1.

Figure 1

(A) Overlay of crystal structures of rabbit ALOX15 (PDB entry 2P0M, conformer A, grey) and human ALOX15B (PDB entry 4NRE, brown). (B) Overlay of crystal structures of rabbit ALOX15 (PDB entry 2P0M, conformer A, grey) and the AA 12-lipoxygenating porcine ALOX15 catalytic domain (PDB entry 3RDE, brown). (C) Structural heterogeneity of rabbit ALOX15 and human ALOX15 B in salt-free aqueous solutions. (C-I) Native polyacrylamide gel electrophoresis (PAGE). For this analysis, the enzymes were prepared and maintained in 20 mM Tris-HCl buffer, pH 8.0 containing 130 mM NaCl. (C-II) Native PAGE. For this analysis, the enzyme was prepared and maintained in 20 mM Tris-HCl buffer, pH 8.0 that does not contain NaCl (desalted enzyme preparation eluted as a single peak by and size-exclusion chromatography). (C-III) Denaturing PAGE. For this analysis the enzyme was prepared and maintained in 20 mM Tris-HCl buffer, pH 8.0, but the running buffer contained 0.5% SDS.