Figure 5.

Identification of SUMO-interacting proteins by SUMO-ID. The N-terminal Split-TurboID construct is fused to the SUMO of interest, and the C-terminal fragment is fused to a potentially SUMOylated (or SIM-containing) target. Target SUMOylation, or SUMO-SIM interaction, results in reconstitution of the Split-TurboID and permits biotin labeling of proximal proteins. Biotinylated proteins can then be purified on streptavidin matrices and analyzed by mass spectrometry (MS). Concept developed in [68].