Figure 2.

Biochemical characterization of the conjugates. (A) Analysis of the anti-HER2 affibody molecules equipped with a single mcDM1 drug molecule and the non-toxic control by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions. The numbers to the left indicate the molecular weights of the marker proteins in lane M (kDa). (B) Analysis of the conjugates by size-exclusion chromatography under native conditions. The numbers above the chromatograms indicate the elution volumes of protein standards with different molecular weights (kDa). (C) Analysis of the conjugates by reversed-phase high-performance liquid chromatography (RP-HPLC). The conjugates were eluted with a 30 min linear gradient from 30 to 60% acetonitrile in water supplemented with 0.1% trifluoroacetic acid (TFA).