Table 1.

Kinetic parameters and equilibrium dissociation constants.

Analytes	Ligand	ka (M−1·s−1) a	kd (s−1) b	KD (M) c
ZHER2–ABD–mcDM13	HER2	3.4 × 104	3.0 × 10−4	8.9 × 10−9
	HSA	3.0 × 105	6.0 × 10−5	2.0 × 10−10
	MSA	5.2 × 105	3.0 × 10−3	5.8 × 10−9
ZHER2–ABD–mcDM1	HER2	3.1 × 105	2.1 × 10−4	6.9 × 10−10
	HSA	1.5 × 106	5.7 × 10−5	3.8 × 10−11
	MSA	2.4 × 106	2.2 × 10−3	9.4 × 10−10
ZHER2–ABD–AA3	HER2	1.0 × 106	3.0 × 10−4	3.0 × 10−10
	HSA	1.5 × 106	7.7 × 10−5	5.2 × 10−11
	MSA	2.7 × 106	2.7× 10−3	1.0 × 10−9

^a association rate constant; ^b dissociation rate constant; ^c equilibrium dissociation constant.