Table 1.
Genes of the core oligosaccharide (core-OS) cluster and putative function of the encoded enzymes in Pseudomonas syringae pv. tomato DC3000 in comparison to P. aeruginosa PAO1 based on publicly available data.
| Identifier | Annotation 1 | Putative function in Pst DC3000 | PAO1 Equivalent | Putative/Proven Function in PAO1 | Identity % |
|---|---|---|---|---|---|
| Genes within core-OS cluster | |||||
| PSPTO_4983 | Lipopolysaccharide biosynthesis protein RfaE | Heptose biosynthesis | hldE (PA4996) | Heptose biosynthesis | 86.9 |
| PSPTO_4984 | Lipid A ABC transporter, ATP-binding/permease protein | Lipid-A:core-OS transport | msbA (PA4997) | Lipid-A:core-OS transport | 83.8 |
| PSPTO_4985 | Toluene tolerance protein | Unknown | PA4998 | Kinase | 55.6 |
| PSPTO_4986 | Membrane protein | Putative OPS ligase | waaL (PA4999) 2 | OPS ligase | No hit/19.4 3 |
| PSPTO_4987 | Hypothetical protein | WbcX-like glycosyltransferase | wapR (PA5000) 2 | Glycosyltransferase (Rha) | No hit/7.9 3 |
| PSPTO_4988 | Hypothetical protein | RfaB family glycosyltransferase | PA5001 | Glycosyltransferase | 73.6 |
| PSPTO_4989 | Hypothetical protein | PIG-L family deacetylase | PA5002 | Unknown | 65.5 |
| PSPTO_4990 | Hypothetical protein | GNAT family N-acetyltransferase | PA5003 | Unknown | 69.1 |
| PSPTO_4991 | Glycoside hydrolase family protein | Glycosyltransferase (GlcII) | wapH (PA5004) | Glycosyltransferase (GlcII) | 71.2 |
| PSPTO_4992 | Carbamoyltransferase family protein | Cm-(→7) carbamoyltransferase | wapO (PA5005) | Carbamoyltransferase | 86.5 |
| PSPTO_4993 | Hypothetical protein | Type III effector HopAC1 (segment) | No hit | ||
| PSPTO_4994 | ISPsy5, transposase | ISPsy5, transposase | No hit | ||
| PSPTO_4995 | ISPsy5, Orf1 | ISPsy5, Orf1 | No hit | ||
| PSPTO_4996 | Hypothetical protein | Type III effector HopAC1 (segment) | No hit | ||
| PSPTO_4997 | Hypothetical protein | Unknown | PA5006 | Kinase | 67.7 |
| PSPTO_4998 | Lipopolysaccharide biosynthesis protein | Heptose kinase | wapQ (PA5007) | Heptose kinase | 57.5 |
| PSPTO_4999 | Lipopolysaccharide core biosynthesis protein | Heptose kinase | wapP (PA5008) | Heptose kinase | 76.2 |
| PSPTO_5000 | Lipopolysaccharide core biosynthesis protein WaaP | Heptose kinase | waaP (PA5009) | Heptose kinase | 79.5 |
| PSPTO_5001 | Lipopolysaccharide core biosynthesis protein WaaG | α-GalN-(1→3) Glycosyltransferase | wapG (PA5010) | Glycosyltransferase (GalN) | 78.6 |
| PSPTO_5002 | Lipopolysaccharide heptosyltransferase | α-HepI-(1→5) Glycosyltransferase | waaC (PA5011) | Glycosyltransferase (HepI) | 74.9 |
| PSPTO_5003 | ADP-heptose--LPS heptosyltransferase II | α-HepII-(1→3) Glycosyltransferase | waaF (PA5012) | Glycosyltransferase (HepII) | 83.5 |
| Genes outside of the cluster | |||||
| PSPTO_1330 | Glycosyltransferase family protein | Glycosyltransferase α-l-Rha-(1→6) | migA (PA0705) | Glycosyltransferase (Rha1→6) | 63.9 |
| PSPTO_2767 | Lipopolysaccharide core biosynthesis domain protein | No hit | |||
1 according to www.pseudomonas.com accessed on 11.01.2021; 2 located outside of the cluster; 3 BLAST yielded no hit, identity result from pairwise alignment of syntenic gene sequences.