Table 6.
Mass spectrometric analysis of core-OS of Pst DC3000 ΔwbpL and wild type. Summary of calculated and observed monoisotopic neutral masses [Da] is given. Accuracy of the measurement is stated as Δppm; n.d. = not detected; * detected, but only with <5% of relative intensity to the major base peak.
| Molecule | Pst DC3000 ΔwbpL | Pst DC3000 WT | |||
|---|---|---|---|---|---|
| Calculated Mass [Da] | Observed Mass [Da] | Error [Δppm] | Observed Mass [Da] | Error [Δppm] | |
| Kdo1Hep1HepCm1Hex2HexN2Ala1Ac1P3–H2O | 1646.381 | 1646.383 * | 1.2 | 1646.384 | 1.8 |
| Kdo1Hep1HepCm1Hex2HexN2Ala1Ac1P3 | 1664.390 | 1664.394 * | 2.4 | 1664.394 | 2.4 |
| Kdo1Hep1HepCm1Hex26dHex1HexN1Ala1Ac2P3–H2O | 1673.380 | 1673.383 * | 1.8 | 1673.384 | 2.4 |
| Kdo1Hep1HepCm1Hex26dHex1HexN1Ala1Ac2P3 | 1691.391 | 1691.393 | 1.2 | 1691.395 | 2.4 |
| Kdo1Hep1HepCm1Hex2HexN2Ala1Ac1P4–H2O | 1726.347 | 1726.349 | 1.2 | 1726.350 | 1.7 |
| Kdo1Hep1HepCm1Hex26dHex1HexN1Ala1Ac1P4 | 1729.347 | 1729.348 | 0.6 | n.d. | - |
| Kdo1Hep1HepCm1Hex2HexN2Ala1Ac1P4 | 1744.358 | 1744.360 | 1.1 | 1744.361 | 1.7 |
| Kdo1Hep1HepCm1Hex26dHex1HexN1Ala1Ac2P4 | 1771.357 | 1771.359 | 1.1 | 1771.361 | 2.3 |
| Kdo1Hep1HepCm1Hex26dHex1HexN2Ala1Ac3P2–H2O | 1796.494 | n.d. | - | 1796.497 | 1.7 |
| Kdo1Hep1HepCm1Hex26dHex1HexN2Ala1Ac3P2 (M2P) | 1814.504 | 1814.509 * | 2.8 | 1814.508 | 2.2 |
| Kdo1Hep1HepCm1Hex26dHex1 HexN2Ala1Ac2P3–H2O | 1834.449 | 1834.452 * | 1.6 | 1834.453 | 2.2 |
| Kdo1Hep1HepCm1Hex26dHex1HexN1Ala1Ac2P5 | 1851.324 | 1851.326 | 1.1 | n.d. | - |
| Kdo1Hep1HepCm1Hex26dHex1HexN2Ala1Ac2P3 | 1852.460 | 1852.462 | 1.1 | 1852.463 | 1.6 |
| Kdo1Hep1HepCm1Hex26dHex1 HexN2Ala1Ac3P3–H2O | 1876.460 | 1876.462 | 1.1 | 1876.463 | 1.6 |
| Kdo1Hep1HepCm1Hex26dHex1HexN2Ala1Ac3P3 (M3P) | 1894.470 | 1894.472 | 1.1 | 1894.474 | 2.1 |
| Kdo1Hep1HepCm1Hex26dHex1 HexN2Ala1Ac2P4–H2O | 1914.416 | 1914.418 | 1.0 | 1914.419 | 1.6 |
| Kdo1Hep1HepCm1Hex26dHex1HexN2Ala1Ac2P4 | 1932.426 | 1932.428 | 1.0 | 1932.430 | 2.1 |
| Kdo1Hep1HepCm1Hex26dHex1 HexN2Ala1Ac3P4–H2O | 1956.426 | 1956.428 | 1.0 | 1956.429 | 1.5 |
| Kdo1Hep1HepCm1Hex26dHex1HexN2Ala1Ac3P4 (M4P) | 1974.437 | 1974.439 | 1.0 | 1974.440 | 1.5 |
| Kdo1Hep1HepCm1Hex26dHex1 HexN2Ala1Ac2P5–H2O | 1994.382 | 1994.384 | 1.0 | 1994.384 | 1.0 |
| Kdo1Hep1HepCm1Hex26dHex1HexN2Ala1Ac2P5 | 2012.393 | 2012.395 | 1.0 | 2012.392 | −0.5 |
| Kdo1Hep1HepCm1Hex26dHex1 HexN2Ala1Ac3P5–H2O | 2036.393 | 2036.395 | 1.0 | 2036.396 | 1.5 |
| Kdo1Hep1HepCm1Hex26dHex1HexN2Ala1Ac3P5 (M5P) | 2054.403 | 2054.405 | 1.0 | 2054.406 | 1.5 |
| Kdo1Hep1HepCm1Hex26dHex1HexN2Ala1Ac2P6–H2O | 2074.348 | n.d. | - | 2074.343 | −2.4 |
| Kdo1Hep1HepCm1Hex26dHex1HexN2Ala1Ac2P6 | 2092.359 | 2092.360 | 0.5 | 2092.353 | −3.3 |
| Kdo1Hep1HepCm1Hex26dHex1 HexN2Ala1Ac3P6–H2O | 2116.359 | 2116.361 | 0.9 | n.d. | - |
| Kdo1Hep1HepCm1Hex26dHex1HexN2Ala1Ac3P6 (M6P) | 2134.369 | 2134.371 | 0.9 | n.d. | - |