Figure 3.

SIN-1 alters the glycosylation pattern of COX-2. HUVEC were incubated for 18 h with SIN-1 alone or in combination with IL-1α. (A) After immunoprecipitation, endoglycosidase H, which cleaves high-mannose-type and hybrid oligosaccharide chains, or PNGase F, which cleaves N-linked tri- and tetraantennary complex-type chains, were added. (B) Incubation of cells is performed in the presence of tunicamycin. COX-2 expression was evaluated by Western blot analysis. β-actin was used as a control of protein loading. The lanes were run on the same gels but were non-contiguous. Results are representative of 3 independent experiments.