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. 2021 Mar 23;10(3):496. doi: 10.3390/antiox10030496

Figure 6.

Figure 6

SIN-1 inhibits the prostaglandin production. HUVEC were treated for 18 h with SIN-1 or IL-1α either alone or in combination. (A) 6-keto-PGF1α production was measured after the exposure of cells to 10 μM arachidonic acid for 30 min. n = 17 independent experiments; *** p < 0.001 vs. unstimulated cells; § p < 0.001vs. IL-1α stimulated cells). (B) Prostaglandin production was measured after the exposure of cells to 2 μM calcium ionophore A23187 for 30 min; n = 5; ** p < 0.01 vs. unstimulated cells; # p < 0.01vs. IL-1α. (C) HUVEC were incubated with SIN-1 or IL-1α in the presence or absence of different concentration of tunicamycin for 6 h and Western blot analysis was performed. The lanes were run on the same gel but were non-contiguous. Results are representative of 4 independent experiments. 6-keto-PGF1α levels were measured by EIA.