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COX-2 activity is partially recovered after immunoprecipitation of the enzyme. HUVEC were treated with SIN-1 or IL-1α either alone or in combination for 18 h and then COX-2 was immunoprecipitated. PGE2 production was measured after the exposure of immunoprecipitates to 25 μM arachidonic acid for 10 min and determined by EIA. n = 6 independent experiments; *** p < 0.001 vs. unstimulated cells.
