Figure 6.

Cell death assay. Differentiated SHSY5Y cells were incubated for three days in the presence of phosphate buffer, or 10 µM 2 h UV-exposed dGAE, 10 µM three days/37 °C-incubated dGAE control, [1-10/Cu²⁺] sample and [1-10/Cu²⁺ H₂0₂] sample. 1 h incubation of cells with 2 mM H₂0₂ was used as a positive control. At the end of the incubation period, cells were incubated with ReadyProbes reagent for 15 min, imaged at 37 °C and 5% CO₂ using Operetta CLS high-content analysis system, and at least 5000 dead and live cells were used for analysis. Only cells treated with 2 mM H₂0₂ showed a significant % of dead cells compared to control. *** indicates p value < 0.001.