Table 3.
List of primers.
| Genes | Forward Primers (5′-3′) | Reverse Primers (5′-3′) |
|---|---|---|
| Scd1 | CAGGAGGGCAGGTTTCCAAG | CGTTCATTTCCGGAGGGAGG |
| Fads1 | CGCCAAACGCGCTACTTTAC | CCACAAAAGGATCCGTGGCA |
| Fads2 | CGTGGGCAAGTTCTTGAAGC | TCTGAGAGCTTTTGCCACGG |
| Elovl1 | CCTGAAGCACTTCGGATGGT | TCACTTGCCCGTCCTTCTTC |
| Elovl2 | GTGATGTCCGGGTAGCCAAG | GGACGCGTGGTGATAGACAT |
| Elovl3 | TACTTCTTTGGCTCTCGCCC | AGCTTACCCAGTACTCCTCCA |
| Elovl5 | TGATGAACTGGGTTCCCTGC | CAGCTGCCCTTGAGTGATGT |
| Elovl6 | AGAACACGTAGCGACTCCGA | TCAGATGCCGACCACCAAAG |
Scd1: Stearoyl-CoA desaturase 1; Fads1 and 2: Fatty acid desaturase 1 and 2; Elovl1, 2, 3, 5, and 6: Elongation of very long chain fatty acids protein 1, 2, 3, 5 and 6.