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. 2021 Mar 22;13(3):230. doi: 10.3390/toxins13030230

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of a pre-culture in a wound-like medium (WLM) and 10% glucose combined with sub-minimum inhibitory concentration (MIC) of vancomycin (0.5× MIC) and linezolid (0.5× MIC) on S. aureus biofilm formation after 16 weeks of culture. The kinetics of the early phase of biofilm formation were determined on (A) NSA739; (B) NSA1077; (C) NSA7475; and (D) NSA1385 by the BioFilm ring test^® (BioFilm Control, France). The control corresponds to the evaluation of biofilm formation of strains in Brain Heart Infusion (BHI) medium alone. Dotted horizontal lines: >7, no biofilm; <2, fixed biofilm. Means ± standard errors of the mean of biofilm indexes (BFIs) for at least three independent replicates are presented. Statistical differences between the different culture conditions at each time were obtained using two-way analysis of variance (ANOVA), followed by Dunnett’s multiple-comparison test.

Effects of a pre-culture in a wound-like medium (WLM) and 10% glucose combined with sub-minimum inhibitory concentration (MIC) of vancomycin (0.5× MIC) and linezolid (0.5× MIC) on S. aureus biofilm formation after 16 weeks of culture. The kinetics of the early phase of biofilm formation were determined on (A) NSA739; (B) NSA1077; (C) NSA7475; and (D) NSA1385 by the BioFilm ring test^® (BioFilm Control, France). The control corresponds to the evaluation of biofilm formation of strains in Brain Heart Infusion (BHI) medium alone. Dotted horizontal lines: >7, no biofilm; <2, fixed biofilm. Means ± standard errors of the mean of biofilm indexes (BFIs) for at least three independent replicates are presented. Statistical differences between the different culture conditions at each time were obtained using two-way analysis of variance (ANOVA), followed by Dunnett’s multiple-comparison test.