Table 2.
Phenotypical modifications of S. aureus cultivated in an in vitro wound-like medium (WLM) mimicking the conditions encountered in chronic wounds and with the addition of other stress parameters (high glucose concentration, addition of antibiotics) for 16 weeks. Bold results are statistically significant (p < 0.01) compared with WLM alone.
| Strains | In Vitro WLM Alone | WLM + 10% Glucose |
WLM + Vancomycin |
WLM + Linezolid |
WLM + 10% Glucose + Vancomycin |
WLM + 10% Glucose + Linezolid |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| β-hemol 1 | SCV 2 | β-hemol | SCV | β-hemol | SCV | β-hemol | SCV | β-hemol | SCV | β-hemol | SCV | |
| NSA739 | 99% ± 2 | 2% ± 2 | 12% ± 5 | 22% ± 2 | 93% ± 5 | 2% ± 2 | 28% ± 4 | 5% ± 2 | 5% ± 2 | 30% ± 7 | 13% ± 6 | 18% ± 7 |
| NSA1077 | 98% ± 3 | 2% ± 2 | 5% ± 5 | 11% ± 2 | 91% ± 7 | 2% ± 3 | 55% ± 5 | 20% ± 5 | 8% ± 4 | 18% ± 5 | 10% ± 10 | 10% ± 5 |
| NSA7475 | 98% ± 2 | 1% ± 2 | 65% ± 3 | 24% ± 2 | 88% ± 7 | 4% ± 2 | 48% ± 2 | 15% ± 4 | 50% ± 5 | 15% ± 3 | 37% ± 8 | 17% ± 8 |
| NSA1385 | 99% ± 2 | 0% ± 2 | 93%± 7 | 4% ± 2 | 100% ± 2 | 0% ± 2 | 75% ± 5 | 7% ± 2 | 94% ± 2 | 2% ± 2 | 91% ± 5 | 3% ± 3 |
1 β-hemolysis. 2 SCV, small colony variant. % corresponds to a mean of counts (±standard deviation) performed on three independent experiments on an average of 200 colonies.