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. 2021 Mar 23;10(3):250. doi: 10.3390/biology10030250

Figure 2.

Figure 2

Proteome profiles and quantification of proteins among different berberine treatment conditions in HCT116 and DLD1 colon cancer cell lines. (A) Workflow of the proteomic study. Two colon cancer cell lines, HCT116 and DLD1, were separately treated with different concentrations of berberine for 48 h, and the control (0 µM) was added with same amount of dimethyl sulfoxide. The total proteins were then extracted, trypsin-digested, and underwent triplicate LC-MS/MS runs for label-free quantitative proteomics analysis. (B) Heatmap of quantified proteins in HCT116 and DLD1 cells. Cells were treated with different concentrations (0 µM, 20 µM, 40 µM) of berberine for 48 h. Groups indicate three technical repeats under each treatment condition; the color bar indicates the Z-score of intensities. (C) Ratio distributions of quantified proteins among technical replicates of each sample.