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. 2021 Mar 23;9(3):667. doi: 10.3390/microorganisms9030667

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Heat resistance test and DPA content of B. subtilis spores. The standard deviation is shown in the graphs. Statistical significance was determined using Student’s t-test. ns, not significant. (A) Numbers of colonies formed on LB agar plates of B. subtilis spores wet heat treated at 85 ℃ and 98 ℃ for 10 min. (B) Amount of CaDPA released by B. subtilis spores that were autoclaved at 121 ℃. The amount of CaDPA released by spores was calculated as % of CaDPA released by B. subtilis PY79. (C) CaDPA released by B. subtilis spores heat treated at 98 ℃ for 1–6 h. The amount of CaDPA released by spores was calculated as % of the total CaDPA.