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. 2021 Mar 22;22(6):3227. doi: 10.3390/ijms22063227

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Altered phosphorylation of lipid metabolic pathways including lipogenesis and lipolysis in SC and OM AT of dairy cows supplemented with CLA. Enzymes with increased or decreased phosphorylation upon CLA supplementation were mapped to lipid metabolism networks. Additionally, the endocannabinoid system (ECS) mechanism was linked to lipolysis based on the literature. Enzymes and metabolites were represented in circles and rectangles respectively. Altered enzymes in cytoplasm shown (purple), in mitochondria (dark blue); and in lipid droplets (brown); metabolites of lipogenesis in mitochondria (orange) and in cytoplasm (green); metabolites of lipolysis in lipid droplets (grey color).