Figure 2.

Effect of temperature, pH and disulfide bond on the antibacterial activity of rCrus1. (A) Micrococcus luteus was incubated with or without (control) rCrus1 (2.5 µM) at various temperatures for 2 h, and bacterial survival was determined by plate count. (B) M. luteus was incubated with or without (control) rCrus1 (2.5 µM) at various pH for 2 h, and bacterial survival was determined as above. (C) Bacteria were incubated with or without (control) rCrus1 (2.5 µM), DTT-treated rCrus1 (2.5 µM), or DTT for 2 h, and bacterial survival was determined as above. Values are shown as means ± SD (N = 3). N, the number of replicates. ** p < 0.01, * p < 0.05 (Student’s t test).