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. 2021 Mar 23;19(3):176. doi: 10.3390/md19030176

Figure 5.

Figure 5

The effect of rCrus1 on bacterial cell membrane integrity. (A) Bacillus cereus and Micrococcus luteus were incubated with rCrus1 or PBS for 2 h. The cells were stained with PI and observed for PI uptake (upper panels) with a fluorescence microscope. The bright field image is shown in the lower panels. (B) B. cereus and M. luteus were pre-incubated with DiSC3(5) and then treated with rCrus1, valinomycin, or PBS, and the fluorescence of the cells was subsequently determined.