KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Phospho-Rb (Ser807/811) (D20B12) | Cell Signaling Technology | RRID:AB_11178658 Cat#8516 |
| Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) | Cell Signaling Technology | RRID:AB_2687505 Cat#11818 |
| Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) | Cell Signaling Technology | RRID:AB_10694233 Cat#5364 |
| Phospho-4E-BP1 (Thr37/46) (236B4) | Cell Signaling Technology | RRID:AB_560835 Cat#2855 |
| Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) | Cell Signaling Technology | RRID:AB_2315112 Cat#4370 |
| GFP (4B10) | Cell Signaling Technology | RRID:AB_1196614 Cat#2955 |
| Goat anti-Rabbit IgG Alexa 647 | Life Technologies | RRID:AB_2535813 A-21245 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Oligomycin A | Sigma | Cat#75351 |
| Insulin | Sigma | Cat#I9278 |
| EGF | Peprotech | Cat#100-15 |
| Hydrocortisone | Sigma | Cat#H0888 |
| Cholera Toxin | Sigma | Cat#C8052 |
| DMEM/F12 | Invitrogen | Cat#11330-032 |
| Pen/Strep | Invitrogen | Cat#15070-063 |
| Antimycin A | Sigma | Cat#A8674 |
| Rotenone | Sigma | Cat#45656 |
| FCCP | Sigma | Cat#C2920 |
| Glucose | Fisher | Cat#D16 |
| MK2206 | Selleckchem | Cat#S1078 |
| Torin1 | Selleckchem | Cat#S2827 |
| 2DG | Sigma | Cat#D8375 |
| 3PO | Selleckchem | Cat#S7639 |
| BU99006 | Santa Cruz Biotechnology | Cat#SC-300307 |
| CAS-648926-15-2 | EMD Millipore | Cat#361515 |
| Etomoxir | Sigma | Cat#E1905 |
| 6AN | Cayman Chemical | Cat#10009315 |
| Cycloheximide | EMD Millipore | Cat#239763 |
| Collagen I, Rat tail | Gibco | Cat#A10483-01 |
| Bafilomycin A | Cayman Chemical | Cat#11038 |
| SBI0206965 | Sigma | Cat#SML1540 |
| PF0175157 | Sigma | Cat#PZ0299 |
| Paraformaldehyde 8% | Electron Microscopy Sciences | Cat#157-8-100 |
| Hoechst 33342 | Life Technologies | Cat#H3570 |
| Cell Culture Media | ||
| DMEM/F-12 1:1 | Life Technologies | Cat#11320 |
| Horse Serum | Invitrogen | 16050-122 |
| DMEM | Life Technologies | 11965175 |
| FBS | Life Technologies | 11965175 |
| Experimental Models: Cell lines | ||
| MCF10A | Janes et al., 2010 | RRID:CVCL_0598 |
| 184A1 | ATCC | RRID:CVCL_3040 |
| MCF7 | ATCC | RRID:CVCL_0031 |
| U87 | ATCC | RRID:CVCL_0022 |
| A549 | ATCC | RRID:CVCL_0023 |
| MCF10A-AMPKAR2 | (Hung et al, 2017) | |
| MCF10A-AMPKAR2-ERKTR | This report | |
| MCF10A-AMPKAR2-TFEBTR | This report | |
| MCF10A-PercevalHR | This report | |
| MCF10A-Ateam | This report | |
| MCF10A-AMPKAR2-GLUT1_NLSmCherry | This report | |
| MCF10A-AMPKAR2-Geminin::mCherry | This report | |
| MCF10A-AMPKAR2-NLS::mCherry | This report | |
| MCF10A-TOP | This report | |
| 184A1-AMPKAR2 | This report | |
| MCF7-AMPKAR2 | This report | |
| U87-AMPKAR2 | This report | |
| A549-AMPKAR2 | This report | |
| Recombinant DNA | ||
| pPBJ-AMPKAR2-puro | (Hung et al, 2017) | |
| pLJM-ERKTR::mCherry | (Sparta et al., 2015) | |
| GW1-PercevalHR | (Tantama et al., 2013) | Addgene#49082 |
| pPBJ-PercevaIHR-puro | This report | |
| ATeam1.03-nD/nA/pcDNA3 | (Imamura et al., 2009) | Addgene#51958 |
| pPBJ-Ateam1.03-puro | This report | |
| TOP-H2B-YFP-DD | (Han et al., 2014) | Addgene#96891 |
| pLJM-geminin::mCherry | (Hung et al., 2017) | |
| pLJM-TFEBTR::mCardinal | This report | |
| pBabe-NLS::mCherry | This report | |
| pBabe-GLUT1-NLS::mCherry | This report | |
| Software and Algorithms | ||
| NIS-Elements AR ver. 4.20 | Nikon | RRID:SCR_014329 |
| Bio-Formats ver. 5.1.1 (May 2015) | OME | RRID:SCR_000450 |
| uTrack 2.0 | (Jaqaman et al., 2008) | http://www.utsouthwestern.edu/labs/danuser/software/ |
| MATLAB | Math works | SCR_001622 |
| Other | ||
| Glass Bottom Plates, #1.5 cover glass | In Vitro Scientific | Cat#P24-1.5H-N, P96-1.5H-N |